Prevalence, genotypes, and risk factors of Enterocytozoon bieneusi in Asiatic black bear (Ursus thibetanus) in Yunnan Province, Southwestern China
| Autor: | Jian-Qiang Han, Jie Wu, Yang Zou, Feng-Cai Zou, Zhao Li, Lian-Qin Shi, Cui-Qin Huang, Jian-Fa Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine China Genotype 030106 microbiology Ursus thibetanus Microsporidiosis Polymerase Chain Reaction 03 medical and health sciences Risk Factors Zoonoses parasitic diseases DNA Ribosomal Spacer medicine Prevalence Animals Enterocytozoon bieneusi Internal transcribed spacer Phylogeny General Veterinary biology General Medicine Enterocytozoon biology.organism_classification medicine.disease Infectious Diseases Insect Science Microsporidia Multilocus sequence typing Parasitology Nested polymerase chain reaction Ursidae Multilocus Sequence Typing |
| Zdroj: | Parasitology research. 117(4) |
| ISSN: | 1432-1955 |
| Popis: | Microsporidiosis is an important zoonotic disease, even leading to severe diarrhea. However, no information about prevalence and genotypes of Enterocytozoon bieneusi infection in Asiatic black bears in southwestern China is available. The objectives of the present study were to investigate the prevalence of E. bieneusi and to characterize their genotypes using the nested PCR amplification of the internal transcribed spacer region of the ribosomal RNA gene cluster and multilocus sequence typing (MLST). The overall prevalence of E. bieneusi was 19.75% (80/405) and the rate of E. bieneusi in Xishuangbanna (33.33%) was significantly higher than that in any other regions (Honghe, 17.65%; Dehong, 13.04%; Kunming, 0; P = 0.01). Sequence analysis revealed that 4 known genotypes (D, n = 2; SC02, n = 10; SC01, n = 5; and CHB1, n = 4) and 13 novel genotypes (designed MJ1–MJ13) were identified. When 17, 5, 14, and 34 sequences at loci MS1, MS3, MS4, and MS7 via MLST analyses, representing 4, 4, 5, and 10 genotypes, respectively, were completed, one multilocus genotype (MLG novel-ABB1) was identified. This is the first report of E. bieneusi in Asiatic black bear in Yunnan province, Southwestern China. The results indicated the potential zoonotic risk of this parasite through the Asiatic black bear in this region and provided foundation data for preventing and controlling E. bieneusi infection of many other animals and humans in these regions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink