Sorting specimen-rich invertebrate samples with cost-effective NGS barcodes: Validating a reverse workflow for specimen processing
| Autor: | Wendy Y. Wang, Rudolf Meier, Seiki Yamane, Amrita Srivathsan, Maosheng Foo |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sample (material) Computational biology Biology Barcode 010603 evolutionary biology 01 natural sciences DNA barcoding Workflow law.invention 03 medical and health sciences symbols.namesake law Databases Genetic Genetics Animals DNA Barcoding Taxonomic Cluster analysis Specimen processing Ecology Evolution Behavior and Systematics Sanger sequencing Ants Sorting Biodiversity Sequence Analysis DNA Classification Invertebrates 030104 developmental biology symbols Biotechnology |
| Zdroj: | Molecular Ecology Resources. 18:490-501 |
| ISSN: | 1755-098X |
| DOI: | 10.1111/1755-0998.12751 |
| Popis: | Biologists frequently sort specimen-rich samples to species. This process is daunting when based on morphology, and disadvantageous if performed using molecular methods that destroy vouchers (e.g., metabarcoding). An alternative is barcoding every specimen in a bulk sample and then presorting the specimens using DNA barcodes, thus mitigating downstream morphological work on presorted units. Such a "reverse workflow" is too expensive using Sanger sequencing, but we here demonstrate that is feasible with an next-generation sequencing (NGS) barcoding pipeline that allows for cost-effective high-throughput generation of short specimen-specific barcodes (313 bp of COI; laboratory cost |
| Databáze: | OpenAIRE |
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