Immunogenicity of a new allergoid from Felis domesticus
| Autor: | A. Cerezo Abellán, E. Bravo Hernández, J.P. Sola González, M. Peñalver-Mellado |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Allergen immunotherapy Dander Injections Subcutaneous Immunology medicine.disease_cause Immunoglobulin E Mice 03 medical and health sciences Immunogenicity Vaccine 0302 clinical medicine Allergen Fel d 1 Allergoids Hypersensitivity medicine Animals Humans Immunology and Allergy Glycoproteins Skin Tests biology business.industry Immunogenicity General Medicine Disease Models Animal Allergoid 030228 respiratory system Desensitization Immunologic Immunoglobulin G Cats biology.protein Female Antibody business 030215 immunology |
| Zdroj: | Allergologia et Immunopathologia. 48:612-618 |
| ISSN: | 0301-0546 |
| DOI: | 10.1016/j.aller.2020.02.008 |
| Popis: | Background The chemical modification of allergens with glutaraldehyde improves safety while maintaining clinical efficacy, which permits the administration of higher doses of immunotherapy, reducing the risk of adverse reactions. The aim of this study is to evaluate the immunogenic capacity of a new cat dander polymer by immunizing mice and quantifying immunoglobulin s in serum, in comparison with the non-modified allergen. Methods The study consists of the immunization of three mice groups with the polymerized and the native extract, together with a negative control group. The immunoglobulin levels in serum have been measured by indirect ELISA. By means of the non-parametric Mann–Whitney U test, it was determined if there were significant differences in the values of specific antibodies between groups. Results The group immunized with the allergoid showed significantly higher specific IgG and IgG1 values to dander allergens and specific IgG to the major allergen Fel d 1, while there were no significant changes in IgG2a and IgE values. These results could be due to a higher immunization dose. The vaccine formulation was based on the optimal defined dose for clinical efficacy of allergen immunotherapy. Conclusions This preclinical study carried out with the present assay has established that the allergoid of cat dander extract, as designed for its optimal use in allergen immunotherapy, produces a higher specific IgG than the native extract, in addition to showing significantly higher specific IgG1 levels, evidencing a greater effectiveness in immunization. |
| Databáze: | OpenAIRE |
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