Structure and stability of warfarin-sodium inclusion complexes formed with permethylated monoamino-β-cyclodextrin
| Autor: | Krisztina Németh, Réka Mizsei, Laszlo Jicsinszky, Júlia Visy, Miklós Simonyi, Gábor Tárkányi, Julianna Szemán, Lajos Szente, Orsolya Tőke |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Aqueous solution Cyclodextrin Stereochemistry Warfarin Sodium beta-Cyclodextrins Clinical Biochemistry Carbohydrates Diastereomer Pharmaceutical Science Hydrogen-Ion Concentration Analytical Chemistry Structure-Activity Relationship Crystallography Drug Stability chemistry Drug Discovery Proton NMR Side chain Warfarin Enantiomer Two-dimensional nuclear magnetic resonance spectroscopy Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 72:292-298 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2012.09.003 |
| Popis: | Inclusion complexes of warfarin enantiomers with permethylated monoamino-β-cyclodextrin (PMMABCD) were characterized using CE and (1)H NMR spectroscopy in aqueous solution. These techniques gave complementary information on the stability and the structure of the diastereomeric host-guest inclusion complexes. The stability constants were determined from CE experiments in a wide pH range. Change in the migration order on the variation of the pH was observed. (1)H NMR assignments have been established for the seven non-equivalent carbohydrate units of the host in the complex at pH 7-9. Specific H-H distance restraints were obtained from NOESY experiments and were introduced into molecular modeling to establish the geometry of the inclusion complexes. It was found that the open side chain warfarin enters the cavity from the primary side of the CD. The orientation of the coumarin ring within the cavity has the same preference for the two warfarin enantiomers owing to an ionic interaction with the amino group of the CD. Accordingly, enantioselectivity at pH 8.5 arises from the difference in the CH/π interactions between warfarin aromatics and the manifold of CH groups of the CD. |
| Databáze: | OpenAIRE |
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