Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides
| Autor: | William C. Davis, Mustafa K. Aydintug, Thomas J. Inzana, Lynette B. Corbeil, Thomas Letonja |
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| Rok vydání: | 1989 |
| Předmět: |
Lipopolysaccharides
Gram-negative bacteria medicine.drug_class Blotting Western Antibodies Heterophile Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody medicine.disease_cause Cross-reactivity Microbiology Lipid A Antibody Specificity Gram-Negative Bacteria medicine Escherichia coli Immunology and Allergy Antigens Bacterial biology Antibodies Monoclonal biology.organism_classification Enterobacteriaceae Antibodies Bacterial Infectious Diseases biology.protein lipids (amino acids peptides and proteins) Antibody Bacteria |
| Zdroj: | The Journal of infectious diseases. 160(5) |
| ISSN: | 0022-1899 |
| Popis: | A panel of eight murine monoclonal antibodies (MAbs) were produced against heat-killed Escherichia coli J5 and shown to react with J5 lipopolysaccharide (LPS) by enzyme-linked immunosorbent assay (ELISA). These antibodies were then assayed by a suspension ELISA for reactivity with up to 20 heterologous smooth or rough isolates of gram-negative bacteria, which were assayed after heat or formalin treatment, or as live cells. Extracted LPS from the same bacteria were tested for reactivity with the MAbs by direct ELISA. The MAbs demonstrated broad cross-reactivity with most heat-treated bacteria. In contrast, cross-reactivity of the MAbs with live or formalin-treated bacteria was limited almost exclusively to E. coli J5, Hemophilus species, or rough mutants of Salmonella minnesota. Reactivity with extracted LPSs and lipid A varied considerably depending on the MAb. Further, when Western blotting was used as the assay only four of eight MAbs reacted with J5 LPS, and none of the MAbs reacted with LPS from smooth S. minnesota or any of its rough mutants. Adsorption of the MAbs with acid hydrolyzed, boiled, or live E. coli J5 prior to ELISA of the MAbs with J5 LPS supported evidence that none of the MAbs were specific for lipid A and that reactivity was greater with boiled than with live cells. Thus, the cross-reactivity of antibodies to E. coli J5 LPS is dependent on the physical state of the bacteria or LPS used for assay, the assay used, and the specificity of the antibody. |
| Databáze: | OpenAIRE |
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