IgIII (270-280)-fragment-like H2N-DDSDEEN-COOH peptide modulates N-CAM expression via Ca2+-dependent ERK signaling during 'in vitro neurogenesis'
| Autor: | Antonietta Gentile, Caterina Morabito, Giorgio Fanò, Simone Guarnieri, Kateryna Kolkova, Maria A. Mariggiò |
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| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
Physiology Biology Transfection Biochemistry PC12 Cells Cellular and Molecular Neuroscience Endocrinology Gallic Acid Extracellular Animals Calcium Signaling Protein kinase A Neural Cell Adhesion Molecules Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Neurogenesis Cell Differentiation Transport protein Cell biology Protein Structure Tertiary Rats Enzyme Activation Protein Transport Neural cell adhesion molecule Signal transduction Oligopeptides Intracellular |
| Zdroj: | Peptides. 29(9) |
| ISSN: | 0196-9781 |
| Popis: | The two major isoforms (180 kDa and 140 kDa) of the neural cell adhesion molecule (N-CAM) are crucially involved in neurogenesis and brain repair via activation of the mitogen-activated protein kinase (MAPK) cascade. Modification by glycosylation, and homophilic and heterophilic interactions regulate the function of N-CAM, but little is known about the interplay of these processes. In the neuron-like PC12 cell line, extracellular small acidic peptides have been shown to modulate the expression of N-CAM mRNA and protein and regulate its translocation to the plasma membrane. Among these peptides, a synthetic Ig-III-like short sequence (H2N-DDSDEEN-COOH), designated sSP, was particularly potent. In this study, we analyzed the cross-talk between nerve growth factor (NGF) and extracellular sSP in native and N-CAM-transfected PC12 cells to determine if these systems interact to modulate transduction pathways and regulate early steps of neurogenesis in vitro. Our results indicate that sSP accelerated the phosphorylation of extracellular regulated kinase-1 (ERK1) and -2 (ERK2) and promoted plasma membrane translocation of 180 kDa N-CAM. By stabilizing cell-cell contacts and promoting cell cluster formation, these events, which were mediated via a significant increase in intracellular Ca2+, regulated some of the early stages of the NGF-induced differentiation process. |
| Databáze: | OpenAIRE |
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