Real-time observation of leukocyte–endothelium interactions in tissue-engineered blood vessel
| Autor: | Zaozao Chen, HaYeun Ji, Leigh Atchison, George A. Truskey, Mingqiang Li, Weiqian Jiang, B. Xu, Kam W. Leong, Ji Sun Park, Min Tang, Dantong Huang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell signaling Time Factors Endothelium medicine.medical_treatment Biomedical Engineering Bioengineering Inflammation Cell Communication 02 engineering and technology Biochemistry Article 03 medical and health sciences Tissue engineering Leukocytes medicine Humans Tissue Engineering Tissue Scaffolds Chemistry Arteries General Chemistry Adhesion 021001 nanoscience & nanotechnology Molecular Imaging Cell biology 030104 developmental biology medicine.anatomical_structure Cytokine Endothelium Vascular medicine.symptom 0210 nano-technology Perfusion Blood vessel |
| Zdroj: | Lab on a Chip. 18:2047-2054 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c8lc00202a |
| Popis: | Human cell-based 3D tissue constructs play an increasing role in disease modeling and drug screening. Inflammation, atherosclerosis, and many autoimmune disorders involve the interactions between immune cells and blood vessels. However, it has been difficult to image and model these interactions under realistic conditions. In this study, we fabricated a perfusion and imaging chamber to allow the real-time visualization of leukocyte perfusion, adhesion, and migration inside a tissue-engineered blood vessel (TEBV). We monitored the elevated monocyte adhesion to the TEBV wall and transendothelial migration (TEM) as the TE BV endothelium was activated by the inflammatory cytokine TNF-α. We demonstrated that treatment with anti-TNF-α or an NF-kB signaling pathway inhibitor would attenuate the endothelium activation and reduce the number of leukocyte adhesion (>74%) and TEM events (>87%) close to the control. As the first demonstration of real-time imaging of dynamic cellular events within a TEBV, this work paves the way for drug screening and disease modeling in TEBV-associated microphysiological systems. |
| Databáze: | OpenAIRE |
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