Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
| Autor: | Harmen van der Meer, Edgar J. J. Groenen, E. Gabriele Panarelli, Peter Gast |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Science Analytical chemistry Astrophysics::Cosmology and Extragalactic Astrophysics 010402 general chemistry Kinetic energy 01 natural sciences 030218 nuclear medicine & medical imaging law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Freezing Animals Horses Electron paramagnetic resonance Microwaves Coupling Multidisciplinary Myoglobin Electron Spin Resonance Spectroscopy Correction Liquid nitrogen 0104 chemical sciences Kinetics chemistry Electron paramagnetic resonance spectrometer Medicine Azide Microwave |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 5, p e0232555 (2020) PLoS ONE, 15(5), e0232555 |
| Popis: | We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave frequencies. |
| Databáze: | OpenAIRE |
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