Role of nitric oxide in histamine release from human basophils and rat peritoneal mast cells
| Autor: | B. Y. C. Wan, Andrew Moulson, Frederick L. Pearce, K. H. Peh, El-Sayed K. Assem |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Nitroprusside Sodium chemistry.chemical_element Basophil Pharmacology Arginine Nitric Oxide Guanidines Nitroarginine Antibodies Rats Sprague-Dawley chemistry.chemical_compound Hemoglobins Sodium fluoride medicine Animals Humans p-Methoxy-N-methylphenethylamine Nitric Oxide Donors Histamine H4 receptor Mast Cells Peritoneal Cavity Calcimycin Histamine N-methyltransferase Nitrates Dose-Response Relationship Drug Sodium Nitrite Immunoglobulin E Mast cell Basophils Rats Methylene Blue medicine.anatomical_structure NG-Nitroarginine Methyl Ester chemistry Biochemistry Molsidomine Sodium Fluoride Sodium nitroprusside Histamine medicine.drug |
| Zdroj: | European journal of pharmacology. 425(3) |
| ISSN: | 0014-2999 |
| Popis: | The effects of a range of nitric oxide (NO)-related compounds on histamine release from human basophils and rat peritoneal mast cells were studied. Basal and immunologic histamine releases from human basophils were not affected by N(omega)-nitro-L-arginine, N(omega)-nitro-L-arginine methyl ester, aminoguanidine or methylene blue (all inhibitors of NO production), sodium nitroprusside (an NO donor), L-arginine (a substrate for NO synthase) or D-arginine (the inactive enantiomer of L-arginine). In rat peritoneal mast cells, NO donors such as sodium nitroprusside, sodium nitrite and sodium nitrate, and lipopolysaccharide (an inducer of NO synthase) had little effect on basal histamine release, while 3-morpholino-sydnonimine (SIN-1, an NO donor), L-arginine and D-arginine increased this release by up to threefold. None of the inhibitors of NO production had any striking effect on histamine release induced by anti-rat immunoglobulin E (IgE), compound 48/80, sodium fluoride, phospholipase C, 1,2-dioctanoyl-sn-glycerol or ionophore A23187. However, haemoglobin was found to inhibit histamine release by anti-rat IgE or A23187 by ca. 40%. Alone of the NO donors, low concentrations of L-arginine produced a mild inhibition of histamine release induced by anti-IgE, compound 48/80 and A23187, but not other ligands, while sodium nitroprusside dose-dependently inhibited (by a maximum of ca. 30%) histamine release by anti-rat IgE, sodium fluoride or A23187. Stimulation with a variety of secretagogues or treatment with L-arginine, D-arginine, lipopolysaccharide, SIN-1 or sodium nitroprusside had no effect on NO production. Similarly, L-arginine, D-arginine or sodium nitroprusside did not change intracellular cGMP levels. On the basis of these results, it is suggested that NO does not play a significant role in the modulation of histamine release from human basophils or rat peritoneal mast cells. The effects of L-arginine, D-arginine and sodium nitroprusside may involve mechanisms unrelated to NO. |
| Databáze: | OpenAIRE |
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