Dietary Effect on the Proteome of the Common Octopus (Octopus vulgaris) Paralarvae

Autor: Juan Carlos Navarro, G. Cardenete, Jose Iglesias, Óscar Monroig, Francisco Hontoria, Inmaculada Varó, Juan José Otero, Eduardo Almansa
Přispěvatelé: Ministerio de Economía y Competitividad (España), Generalitat Valenciana, CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Frontiers in Physiology, Vol 8 (2017)
e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía
Popis: Nowadays, the common octopus (Octopus vulgaris) culture is hampered by massive mortalities occurring during early life-cycle stages (paralarvae). Despite the causes of the high paralarvae mortality are not yet well-defined and understood, the nutritional stress caused by inadequate diets is pointed out as one of the main factors. In this study, the effects of diet on paralarvae is analyzed through a proteomic approach, to search for novel biomarkers of nutritional stress. A total of 43 proteins showing differential expression in the different conditions studied have been identified. The analysis highlights proteins related with the carbohydrate metabolism: glyceraldehyde-3-phosphate-dedydrogenase (GAPDH), triosephosphate isomerase; other ways of energetic metabolism: NADP+-specific isocitrate dehydrogenase, arginine kinase; detoxification: glutathione-S-transferase (GST); stress: heat shock proteins (HSP70); structural constituent of eye lens: S-crystallin 3; and cytoskeleton: actin, actin-beta/gamma1, beta actin. These results allow defining characteristic proteomes of paralarvae depending on the diet; as well as the use of several of these proteins as novel biomarkers to evaluate their welfare linked to nutritional stress. Notably, the changes of proteins like S-crystallin 3, arginine kinase and NAD+ specific isocitrate dehydrogenase, may be related to fed vs. starving paralarvae, particularly in the first 4 days of development.
This study was funded by the “Ministerio de Economía y Competividad (Spanish Government)” under the projects OCTOPHYS (AGL-2010-22120-CO3-02) and OCTOWELF (AGL2013-49101-C2-2-R), and by the “Generalitat Valenciana” under the project PROMETEO II/2014/085. We acknowledge support of the publication fee by the CSIC Open Access Support Initiative through its Unit of Information Resources for Research (URICI)
Databáze: OpenAIRE