Amino- and carboxy-terminal domains of the yeast Rab escort protein are both required for binding of Ypt small G proteins
| Autor: | Bettina E. Bauer, Antonella Ragnini, Rudolf J. Schweyen, Duc Minh Bui, Marta Miaczynska, Stefano Lorenzetti |
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| Rok vydání: | 1996 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Molecular Sequence Data Saccharomyces cerevisiae Protein Prenylation Small G Protein yeast Choroideremia GTP Phosphohydrolases Fungal Proteins Rabs Structure-Activity Relationship GTP-Binding Proteins Transferases Heterotrimeric G protein medicine Animals Amino Acid Sequence Settore BIO/10 Molecular Biology Adaptor Proteins Signal Transducing Guanine Nucleotide Dissociation Inhibitors Sequence Deletion Genetics Fungal protein Alkyl and Aryl Transferases biology prenyltransferases yeast intracellular traffic Rabs choroideraemia choroideraemia Cell Biology medicine.disease biology.organism_classification rab GTP-Binding Proteins Essential gene prenyltransferases Mutagenesis Site-Directed Protein prenylation Cattle Rab intracellular traffic Carrier Proteins Protein Processing Post-Translational Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.7.10.1521 |
| Popis: | The Rab escort protein (REP) is an essential component of the heterotrimeric enzyme Rab geranylgeranyl transferase that modifies the carboxy-terminal cysteines of the Ras-like small G proteins belonging to the Rab/Ypt family. Deletions in the human CHM locus, encoding one of the two REPs known in humans, result in a retinal degenerative syndrome called choroideremia. The only known yeast homologue of the choroideremia gene product is encoded by an essential gene called MRS6. Besides three structurally conserved regions (SCRs) previously detected in the amino-terminal half of REPs and RabGDIs, three other regions in the carboxy-terminal domain (RCR 1-3) are here identified as being characteristic of REPs alone. We have performed the first mutational analysis of a REP protein to experimentally define the regions functionally important for Rab/Ypt protein binding, making use of the genetic system of the yeast Saccharomyces cerevisiae. This analysis has shown that the SCRs are necessary but not sufficient for Ypt1p binding by the yeast REP, the carboxy-terminal region also being required. |
| Databáze: | OpenAIRE |
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