Antioxidant and Antibacterial Peptides from Soybean Milk through Enzymatic- and Membrane-Based Technologies
Autor: | Gyula Vatai, Renáta Gerencsér-Berta, Geremew Geidare Kailo, Szilvia Bánvölgyi, Ildikó Galambos, Barbara Csehi, Klára Pásztorné-Huszár, Zsuzsanna Mednyánszky, Gabriella Kiskó, Arijit Nath, Emília Pozsgai |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
antibacterial peptide Bioengineering Membrane bioreactor 01 natural sciences membrane bioreactor lcsh:Technology Article chemistry.chemical_compound Hydrolysis 0404 agricultural biotechnology fluids and secretions 010608 biotechnology Enzymatic hydrolysis Bioreactor lcsh:QH301-705.5 Chromatography lcsh:T soybean milk Substrate (chemistry) food and beverages enzymatic hydrolysis 04 agricultural and veterinary sciences 040401 food science Papain antioxidant peptide Membrane chemistry lcsh:Biology (General) Antibacterial activity |
Zdroj: | Bioengineering, Vol 7, Iss 1, p 5 (2019) Bioengineering Volume 7 Issue 1 |
ISSN: | 2306-5354 |
Popis: | Enzymatic hydrolysis of soybean milk proteins with cysteine protease papain was performed in an advanced bioreactor, operated with batch mode. In soybean milk protein hydrolysis reaction, enzyme and substrate ratio and reaction temperature were varied, ranging from 0.029:100&ndash 0.457:100 and 30&ndash 60 ° C, respectively. The degree of hydrolysis of soybean milk proteins was increased with increase of enzyme and substrate (soybean milk protein) ratio. However, the degree of hydrolysis was increased due to change of reaction temperature from 30 ° C to 60 ° C with enzyme and substrate ratio 0.229:100 and was reduced when hydrolysis reaction was performed with enzyme and substrate ratio 0.11:100 at hydrolysis temperature 60 ° C. Antioxidant capacity of enzyme-treated milk had a similar trend with degree of hydrolysis. In a later exercise, a membrane bioreactor was adopted for continuous production of antioxidant and antibacterial peptides from soybean milk. The membrane bioreactor was operated for 12 h with constant feeding. Ceramic-made tubular membrane with a pore size 20 nm was used. Application of static turbulence promoter in a membrane separation process was investigated and its positive effects, with respect to higher permeate flux and lower energy consumption in filtration process, were proven. Antioxidant capacity and antibacterial activity against Bacillus cereus of enzyme-hydrolyzed milk and permeate from membrane were confirmed. |
Databáze: | OpenAIRE |
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