Phenotyping and auto-antibody production by liver-infiltrating B cells in primary sclerosing cholangitis and primary biliary cholangitis

Autor: Tom H. Karlsen, Gary M. Reynolds, Bardia T. Guevel, D.B.R.K. Gupta Udatha, Zania Stamataki, Evaggelia Liaskou, Brian K. Chung, Eva Kristine Klemsdal Henriksen, Gideon M. Hirschfield
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Pathology
Fluorescent Antibody Technique
Autoimmunity
Plasma cell
medicine.disease_cause
0302 clinical medicine
Immunology and Allergy
B-Lymphocytes
Liver Cirrhosis
Biliary
Middle Aged
3. Good health
medicine.anatomical_structure
Phenotype
Liver
030211 gastroenterology & hepatology
Female
Antibody
Adult
medicine.medical_specialty
Adolescent
Immunology
Cholangitis
Sclerosing
Plasma Cells
chemical and pharmacologic phenomena
Enzyme-Linked Immunosorbent Assay
Human leukocyte antigen
Biology
digestive system
Peripheral blood mononuclear cell
CD19
Primary sclerosing cholangitis
Immunophenotyping
03 medical and health sciences
Young Adult
medicine
Humans
Lymphocyte Count
Aged
Autoantibodies
Autoantibody
medicine.disease
Antigens
CD20
digestive system diseases
030104 developmental biology
Antibody Formation
biology.protein
Biomarkers
Zdroj: Journal of autoimmunity. 77
ISSN: 1095-9157
Popis: Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are immune-mediated biliary diseases that demonstrate prominent and restricted genetic association with human leukocyte antigen (HLA) alleles. In PBC, anti-mitochondrial antibodies (AMA) are specific and used as diagnostic biomarkers. PSC-relevant auto-antibodies remain controversial despite a distinct HLA association that mirrors archetypical auto-antigen driven disorders. Herein, we compared antibody-secreting B cells (ASCs) in PSC and PBC liver explants to determine if liver-infiltrating ASCs represent an opportune and novel source of disease-relevant auto-antibodies. Using enzymatic digestion and mechanical disruption, liver mononuclear cells (LIMCs) were isolated from fresh PSC and PBC explants and plasmablast (CD19+CD27+CD38hiCD138−) and plasma cell (CD19+CD27+CD38hiCD138+) ASCs were enumerated by flow cytometry. We observed 45-fold fewer plasma cells in PSC explants (n = 9) compared to PBC samples (n = 5, p
Databáze: OpenAIRE
Externí odkaz: