Induction of Soluble and Cell Wall Peroxidases by Aphid Infestation in Barley
| Autor: | Luis J. Corcuera, Gustavo E. Zúñiga, Victor H. Argandoña, Liliana Cardemil, Mercedes E. Chaman |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.disease_cause
Isozyme Cell wall Cell Wall parasitic diseases Infestation Botany medicine Animals Poaceae Aphid biology food and beverages Hordeum Aphididae General Chemistry Lice Infestations biology.organism_classification Isoenzymes Peroxidases Biochemistry Aphids biology.protein Hordeum vulgare Isoelectric Focusing General Agricultural and Biological Sciences Peroxidase |
| Zdroj: | Journal of Agricultural and Food Chemistry. 49:2249-2253 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Peroxidase enzymes have been found in soluble, ionically bound, and covalently bound forms and have been implicated in several physiological processes in plants. This paper investigates the effect of aphid infestation on soluble and bound-cell wall peroxidase activity and bound-cell wall isoform changes of barley plants. Peroxidase activity was measured in control plants and plants infested with the aphid Schizaphis graminum (Rondani). The activity of soluble peroxidases increased with time of infestation, older plants being more affected than younger ones. The increase in bound-cell wall peroxidase activity as a function of age was higher in infested than in control plants, being higher in ionically bound than in covalently bound peroxidases. When the aphids were removed from plants, the activities of both types of peroxidases decreased to control levels. Isoelectrofocusing analyses of the ionically bound peroxidases showed changes in the isoform pattern. A new isoform was induced by infestation. The activities of all covalently bound isoforms increased after infestation. The physiological implications of these changes are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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