Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles
| Autor: | Priscila Pires, Cesar Henrique Wanke, Sanna Sillankorva, Júnia Capua de Lima Novello, Otávio Bianchi, Joana Azeredo, Eduardo Cesar Tondo |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
applications packaging 02 engineering and technology 010402 general chemistry 01 natural sciences Seal (mechanical) chemistry.chemical_compound Materials Chemistry Copolymer media_common.cataloged_instance functionalization of polymers European union media_common Science & Technology Horizon (archaeology) Chemistry Ethylene-vinyl acetate General Chemistry 021001 nanoscience & nanotechnology Pulp and paper industry 3. Good health 0104 chemical sciences Surfaces Coatings and Films Mineral water 0210 nano-technology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Pseudomonas aeruginosa has been found in bottled natural mineral water, even though its presence is not allowed in this product by different food regulations. This study aimed to investigate the inactivation of P. aeruginosa present in mineral water by vB_PaeM_CEB_DP1 (short name DP1) bacteriophage immobilized on ethylenevinyl acetate (EVA) copolymer used as seal caps of plastic bottles. EVA was chemically modified using microwaveassisted alcoholysis, improving polymerphage binding. After that, DP1 phage was attached to EVA and EVAOH copolymers and both surfaces were tested for plaque formation using P. aeruginosa. Then, both materials containing immobilized phages were used as seal caps of plastic bottles and its antimicrobial capacity was tested against P. aeruginosa contaminating mineral water. The EVAOH resulted in higher hydrogen bond density that contributed significantly to the phage immobilization on the polymer surface. The polymers containing immobilized phages were able to reduce 0.53 log of P. aeruginosa population present inside mineral water bottles after 14days. The authors thank CAPES for scholarships to Cesar H. Wanke and Junia Novello. CNPq—National Council for Scientific and Technological Development, Brazil for financial support (grant numbers 308241/2015-0 and 306086/2018-2). Sanna Sillankorva acknowledges funding from the European Union’s Horizon 2020 research and innovation programme (grant number 713640). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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