Defective Mismatch-Repair Colorectal Cancer
| Autor: | F. Ignacio Aranda, Artemio Payá, Gloria Peiró, Miguel Pérez-Mateo, Cristina Alenda, Rodrigo Jover, María José Poveda |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Base Pair Mismatch Colorectal cancer Polymerase Chain Reaction Gastroenterology Proto-Oncogene Proteins Internal medicine medicine Humans Ascending colon Stage (cooking) Family history Adaptor Proteins Signal Transducing Aged Neoplasm Staging Aged 80 and over business.industry Nuclear Proteins Microsatellite instability Cancer General Medicine Middle Aged medicine.disease Immunohistochemistry digestive system diseases Neoplasm Proteins DNA-Binding Proteins MutS Homolog 2 Protein Female DNA mismatch repair Carrier Proteins Colorectal Neoplasms MutL Protein Homolog 1 business Microsatellite Repeats |
| Zdroj: | American Journal of Clinical Pathology. 122:389-394 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1309/v9pgk2y260vfvulr |
| Popis: | The purpose of our study was to determine the usefulness of immunohistochemical analysis for the diagnosis of mismatch-repair ( MMR ) gene defective colorectal tumors and to describe their prevalence and clinicopathologic characteristics. We studied 172 cases. DNA was extracted from formalin-fixed, paraffin-embedded surgical samples, and microsatellite analysis was performed by polymerase chain reaction with BAT-26. The results were correlated with immunohisto-chemical analysis for hMLH1 and hMSH2. Microsatellite instability (MSI) was detected in 13 (7.6%) tumors, and all showed loss of protein expression of hMLH1 (11/13) or hMSH2 (2/13) ( P < .000). Patients with MMR -defective tumors more frequently had poorly differentiated tumors (5/13 [38%] vs 18/159 [11.3%]; P = .02) located in the ascending colon (8/13 [62%] vs 30/159 [18.9%]; P < .0001) and a personal history of other neoplasms (4/13 [31%] vs 18/159 [11.3%]; P = .05). There were no differences in age, family history of cancer, or TNM stage. Immunohistochemical analysis seems to be a reliable method to detect most colorectal cancers with defective MMR genes. |
| Databáze: | OpenAIRE |
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