Binding to leukocytes and induction of TNFα production involve different sites within the lipid-A region of LPS-like molecules
| Autor: | Z. Hmama, Jean-Pierre Revillard, Anne Mey, Hans Binz, Gerard Normier |
|---|---|
| Přispěvatelé: | Unité mixte de recherche biologie moléculaire de la cellule, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1994 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Molecular Sequence Data Immunology Biology [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Peripheral blood mononuclear cell Lipid A Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Leukocytes medicine ComputingMilieux_MISCELLANEOUS 030304 developmental biology Whole blood Pharmacology 0303 health sciences Tumor Necrosis Factor-alpha Monocyte food and beverages Ligand (biochemistry) 3. Good health medicine.anatomical_structure Carbohydrate Sequence chemistry Biochemistry 030220 oncology & carcinogenesis Tumor necrosis factor alpha Signal transduction |
| Zdroj: | International Journal of Immunopharmacology International Journal of Immunopharmacology, Elsevier, 1994, 16 (5-6), pp.413-417. ⟨10.1016/0192-0561(94)90030-2⟩ International Journal of Immunopharmacology, 1994, 16 (5-6), pp.413-417. ⟨10.1016/0192-0561(94)90030-2⟩ |
| ISSN: | 0192-0561 |
| Popis: | The induction of TNF alpha synthesis in whole blood culture assay and isolated peripheral blood mononuclear cells was investigated, using LPS from Klebsiella pneumoniae and two water-soluble 34 kDa derivatives designed as acylpolygalactoside (APG) and EFA-APG, an APG molecule bearing two additional ester-linked fatty acids. Both APG and EFA-APG bind to monocytes by specific ligand receptor interaction but only EFA-APG could induce TNF alpha synthesis. It is concluded that ester-linked fatty acids are not involved in LPS binding to the cell surface, but play a critical role in the triggering of cellular responses. |
| Databáze: | OpenAIRE |
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