Self-collected saliva for SARS-CoV-2 detection: A prospective study in the emergency room
| Autor: | Martin Ypas, Pamela Elizabeth Rodríguez, Guadalupe Carballal, Cristina Videla, Matias G Perez, Noelia Reyes, Carmen Ricarte, Marcela Echavarria, Alfredo Martinez, María P Rodriguez, Martin E. Stryjewski, Alejandro Seoane |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Saliva NASOPHARYNGEAL SWAB Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Signs and symptoms SARS-COV-2 medicine.disease_cause Gastroenterology SALIVA Specimen Handling 03 medical and health sciences purl.org/becyt/ford/3.3 [https] 0302 clinical medicine Interquartile range Virology Internal medicine medicine Humans 030212 general & internal medicine Prospective Studies Prospective cohort study Letters to the Editor Letter to the Editor Coronavirus Aged business.industry Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 technology industry and agriculture COVID-19 Middle Aged Confidence interval Infectious Diseases PCR 030211 gastroenterology & hepatology Female purl.org/becyt/ford/3 [https] business Emergency Service Hospital Viral load |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Journal of Medical Virology |
| DOI: | 10.1002/jmv.26839 |
| Popis: | Current diagnostic standards involve severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs (NPS), but saliva is an attractive and noninvasive option for diagnosis. The objectives were to determine the performance of saliva in comparison with NPS for detecting SARS-CoV-2 and to compare the optimized home brew reverse-transcription polymerase chain reaction (RT-PCR) with a commercial RT-PCR. Paired NPS and saliva specimens were prospectively collected and tested by RT-PCR from patients presenting at an emergency room with signs and symptoms compatible with coronavirus disease-2019. A total of 348 samples from 174 patients were tested by RT-PCR assays. Among 174 patients with symptoms, 63 (36%) were SARS-CoV-2 positive in NPS using the optimized home-brew PCR. Of these 63 patients, 61 (98%) were also positive in saliva. An additional positive SARS-CoV-2 saliva was detected in a patient with pneumonia. Kappa Cohen´s coefficient agreement between NPS and saliva was 0.96 (95% confidence interval [CI], 0.90?0.99). Median Ct values in NPS versus saliva were 18.88 (interquartile range [IQR], 15.60?23.58; range, 11.97?38.10) versus 26.10 (IQR, 22.75?30.06; range, 13.78?39.22), respectively (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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