Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells

Autor:	Paul M. Armistead, Steven A. Soper, Victoria L. Bae-Jump, Peter M. Voorhees, Joshua M. Jackson, Mateusz L. Hupert, Małgorzata A. Witek, Sally A. Hunsucker, Travis Sapp, Weiya Z. Wysham, Caroline E. Perry, Paola A. Gehrig, Maria A. M. Lindell, Soumya V. Nair
Rok vydání:	2015
Předmět:	DNA Single-Stranded Antigens CD34 Cleavage (embryo) Article Catalysis chemistry.chemical_compound Circulating tumor cell Antigens Neoplasm Cell Line Tumor Endopeptidases Materials Chemistry Humans Viability assay Uracil Uracil-DNA Glycosidase chemistry.chemical_classification Endodeoxyribonucleases Cell adhesion molecule Serine Endopeptidases Metals and Alloys Antibodies Monoclonal Membrane Proteins General Chemistry Epithelial Cell Adhesion Molecule Neoplastic Cells Circulating Surfaces Coatings and Films Electronic Optical and Magnetic Materials Enzyme chemistry Biochemistry Gelatinases Uracil-DNA glycosylase Ceramics and Composites Cell Adhesion Molecules DNA
Zdroj:	Chemical Communications. 51:3266-3269
ISSN:	1364-548X 1359-7345
Popis:	We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (~90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a430dd94bb713ddb28810feb028b267 https://doi.org/10.1039/c4cc09765c Zobrazit plný text záznamu