The Susceptibility of Mycobacterium tuberculosis to Isoniazid and the Arg→Leu Mutation at Codon 463 of katG Are Not Associated
| Autor: | H. R. van Doorn, A. G. A. Welten, D. van Soolingen, P. E. W. De Haas, E.J. Kuijper, A. van der Ende, Jacob Dankert |
|---|---|
| Přispěvatelé: | Other departments |
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Tuberculosis medicine.drug_class Antibiotics Antitubercular Agents Arginine Polymerase Chain Reaction law.invention Microbiology Mycobacterium tuberculosis Bacterial Proteins Leucine law Isoniazid medicine Humans heterocyclic compounds Codon Polymerase chain reaction Catalase-peroxidase Netherlands biology Drug Resistance Microbial Mycobacteriology and Aerobic Actinomycetes Sequence Analysis DNA respiratory system biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease respiratory tract diseases Restriction enzyme Peroxidases Mutation Mutation (genetic algorithm) medicine.drug |
| Zdroj: | Journal of clinical microbiology, 39(4), 1591-1594. American Society for Microbiology |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.4.1591-1594.2001 |
| Popis: | A mutation (CCG→CTG [Arg→Leu]) in codon 463 of katG (catalase peroxidase) of Mycobacterium tuberculosis has been found in isoniazid (INH)-resistant strains. A PCR restriction endonuclease analysis to detect this mutation was applied to 395 M. tuberculosis isolates from patients in The Netherlands. The proportion of isolates with a detectable mutation was 32% (32 out of 100) and 29% (85 out of 295) among INH-susceptible isolates and INH-resistant or -intermediate isolates, respectively. Sequencing of five INH-susceptible isolates with such mutations showed that all five had the Arg463Leu mutation. We conclude that the Arg463Leu mutation of katG of M. tuberculosis is not a reliable indicator of INH resistance. |
| Databáze: | OpenAIRE |
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