RGS Expression Rate-Limits Recovery of Rod Photoresponses
| Autor: | Desheng Chen, C.M. Krispel, Nidia Quillinan, Kirill A. Martemyanov, Yu Jiun Chen, Nathan A. Melling, Marie E. Burns, Vadim Y. Arshavsky, Theodore G. Wensel, Ching-Kang Chen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Light genetic structures GTPase-activating protein G-Protein-Coupled Receptor Kinase 1 G protein Neuroscience(all) Blotting Western Gene Expression Mice Transgenic In Vitro Techniques Retina Mice 03 medical and health sciences 0302 clinical medicine Retinal Rod Photoreceptor Cells RGS9 Animals Phosphorylation Vision Ocular 030304 developmental biology G protein-coupled receptor 0303 health sciences G protein-coupled receptor kinase biology General Neuroscience GTP-Binding Protein beta Subunits Recovery of Function Cell biology Mice Inbred C57BL Rhodopsin kinase Rhodopsin biology.protein sense organs RGS Proteins Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | Neuron. 51(4):409-416 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2006.07.010 |
| Popis: | SummarySignaling through G protein-coupled receptors (GPCRs) underlies many cellular processes, yet it is not known which molecules determine the duration of signaling in intact cells. Two candidates are G protein-coupled receptor kinases (GRKs) and Regulators of G protein signaling (RGSs), deactivation enzymes for GPCRs and G proteins, respectively. Here we investigate whether GRK or RGS governs the overall rate of recovery of the light response in mammalian rod photoreceptors, a model system for studying GPCR signaling. We show that overexpression of rhodopsin kinase (GRK1) increases phosphorylation of the GPCR rhodopsin but has no effect on photoresponse recovery. In contrast, overexpression of the photoreceptor RGS complex (RGS9-1·Gβ5L·R9AP) dramatically accelerates response recovery. Our results show that G protein deactivation is normally at least 2.5 times slower than rhodopsin deactivation, resolving a long-standing controversy concerning the mechanism underlying the recovery of rod visual transduction. |
| Databáze: | OpenAIRE |
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