Additional file 2 of Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence

Autor: Wang, Dan, Zhang, Dan-Dan, Song, Jian, Li, Jun-Jiao, Wang, Jun, Li, Ran, Klosterman, Steven J., Kong, Zhi-Qiang, Lin, Fa-Zhuang, Dai, Xiao-Feng, Subbarao, Krishna V., Chen, Jie-Yin
Rok vydání: 2022
Předmět:
DOI: 10.6084/m9.figshare.19227136.v1
Popis: Additional file 2: Figure S1. Analysis of the cell death suppression activities of 120 VdSCPs in Nicotiana benthamiana leaves. Figure S2. Analysis of the broad-spectrum cell death suppression activities of CFEM-containing VdSCPs in Nicotiana benthamiana leaves. Figure S3. Analysis of signal peptide function and subcellular localization of VdSCP76 and VdSCP77. Figure S4. Polymerase chain reaction (PCR) analysis of gene deletion transformants of CFEM-containing VdSCPs from V. dahliae. Figure S5. Polymerase chain reaction (PCR) analysis of gene complementation transformants. Figure S6. Identification of the immunity suppression activity of VdSCP76 and VdSCP77 in Nicotiana benthamiana. Figure S7. Immunoblotting analysis of proteins in Nicotiana benthamiana leaves transiently expressing VdEG1 in the suppression experiment. Figure S8. Pathogenicity assay of double gene deletion of VdSCP76 and VdSCP77 strains on cotton. Figure S9. Functional diversification analysis of CFEM-containing VdSCPs family members. Figure S10. Gene expression of CFEM-containing VdSCPs family members during infection of cotton roots. Figure S11. Analysis of defense related-gene expression level. Figure S12. The fungal biomass and disease index of indicated strains on cotton. Figure S13. Functional analyses of conserved asparagine residues in the CFEM domain-containing members from Verticillium dahliae. Figure S14. Gene expression level of CFEM-containing VdSCPs under iron starvation and ferric ion conditions. Figure S15. Functional dissection Asp-type and Asn-type CFEM-containing secretory proteins from Fusarium oxysporum and Magnaporthe oryzae.
Databáze: OpenAIRE