Isolation and characterization of a Chinese hamster lung cell tryptophanyl-tRNA synthetase mutant
| Autor: | John J. Wasmuth, Stephen M. W. Chang |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Mutant
Hamster Tryptophan-tRNA Ligase Biology Chinese hamster Cell Line Amino Acyl-tRNA Synthetases chemistry.chemical_compound Cricetulus Biosynthesis Cricetinae Genetics Animals Gene Lung chemistry.chemical_classification Structural gene Temperature Tryptophan Cell Biology General Medicine biology.organism_classification Amino acid Clone Cells Kinetics chemistry Biochemistry Cell culture Mutation Cell Division |
| Zdroj: | Somatic cell and molecular genetics. 10(2) |
| ISSN: | 0740-7750 |
| Popis: | A novel type of temperature-sensitive protein synthetic mutant was isolated from V-79 Chinese hamster lung cells using an amino acid analog suicide selection. The expression of the temperature sensitive phenotype of the mutant is greatly affected by the concentration of tryptophan in the culture medium. In addition, the activity of tryptophanyl-tRNA synthetase is undetectable in cell-free extracts prepared from the mutant cells. The results suggest that the mutant has an alteration in the structural gene encoding tryptophanyl-tRNA synthetase. |
| Databáze: | OpenAIRE |
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