Optimization of Amine-Rich Multilayer Thin Films for the Capture and Quantification of Prostate-Specific Antigen
| Autor: | Roberta Polak, Scott P. O. Danielsen, Grinia M. Bradwell, Michael F. Rubner, Robert E. Cohen, Marisa Masumi Beppu, Jonathan B. Gilbert |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Nanotechnology Sensitivity and Specificity Allylamine chemistry.chemical_compound Quantum Dots Biomarkers Tumor Polyamines Electrochemistry medicine Humans General Materials Science Particle Size Thin film Spectroscopy chemistry.chemical_classification Molecular Structure Photoelectron Spectroscopy Biomolecule Prostatic Neoplasms Surfaces and Interfaces Prostate-Specific Antigen Condensed Matter Physics chemistry Chemical engineering Quantum dot Ionic strength Biotinylation Polystyrenes Amine gas treating Swelling medicine.symptom |
| Zdroj: | Langmuir. 31:5479-5488 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | It is demonstrated that poly(allylamine hydrochloride)/poly(styrenesulfonate) (PAH/SPS) multilayer films can be successfully tailored for the capture and detection of small biomolecules in dilute concentrations. Based on in vitro results, these films could be potentially applied for rapid and high-throughput diagnosis of dilute biomarkers in serum or tissue. PAH presents functional amino groups that can be further reacted with desired chemistries in order to create customizable and specific surfaces for biomolecule capture. A variety of film assembly characteristics were tested (pH, molecular weight of PAH, and ionic strength) to tune the biotinylation and swelling behavior of these films to maximize detection capabilities. The resultant optimized biotinylated PAH/SPS 9.3/9.3 system was utilized in conjunction with quantum dots (Qdots) to capture and detect a dilute biomarker for prostate cancer, prostate-specific antigen (PSA). Compared to previous work, our system presents a good sensitivity for PSA detection within the clinically relevant range of 0.4-100 ng/mL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|