Epitope-Cavities Generated by Molecularly Imprinted Films Measure the Coincident Response to Anthrax Protective Antigen and Its Segments
| Autor: | Sue-Chen Wang, Hsin-Tzu Liu, Dar-Fu Tai, Kuo-Hao Lu, Yu-Der Lee, Guan-Yu Chen, Ming-Hong Jhang |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Antigens Bacterial biology Molecular mass Stereochemistry Bacterial Toxins Peptide Biosensing Techniques Quartz Quartz crystal microbalance Sensitivity and Specificity Epitope Analytical Chemistry Molecular Imprinting Epitopes Antigen chemistry biology.protein Amino Acid Sequence Peptides Molecular imprinting Furin Peptide sequence |
| Zdroj: | Analytical Chemistry. 82:2290-2293 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | A molecularly imprinted film was fabricated, in the presence of epitope-peptides, onto a quartz crystal microbalance (QCM) chip. These five peptides are known linear or conformational epitopes of the anthrax protective antigen PA(83). Imprinting resulted in an epitope-cavity with affinity for the corresponding template. With the use of a basic monomer, the binding-effect was further enhanced increasing the affinity to nanomolar levels. The affinities of the peptide to their corresponding molecularly induced polymers (MIPs) were more closely related to the molecular weight of the analyte than to the number of residues. All epitope-cavities differentiated their epitope region on the protective antigen PA(83) as well as the corresponding furin cleavage fragments PA(63) and PA(20). The QCM chip differential response to the protective antigen fragment was observed in the picomolar range, thus demonstrating a method to manipulate protein on the surface with defined orientation. |
| Databáze: | OpenAIRE |
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