Standardized human bone marrow-derived stem cells infusion improves survival and recovery in a rat model of spinal cord injury

Autor: G. Brook, L. Jansen, O. Moskvin, Boris W. Kramer, S. Munter, Berta Cillero-Pastor, J. P. J. M. de Munter, J. Beugels, Tatyana Strekalova, D. A. Pavlov, Wolters E.Ch.
Přispěvatelé: Promovendi MHN, Psychiatrie & Neuropsychologie, RS: MHeNs - R3 - Neuroscience, Promovendi NTM, Plastische Chirurgie (PLC), Ondersteunend personeel NTM, Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS), Kindergeneeskunde, MUMC+: MA Medische Staf Kindergeneeskunde (9)
Rok vydání: 2019
Předmět:
Male
Time Factors
CYCLOSPORINE-A
Interleukin-1beta
CD34
LOCAL-DELIVERY
Apoptosis
Pharmacology
0302 clinical medicine
030212 general & internal medicine
Gliosis
Spinal cord injury
Bone Marrow Transplantation
Resolution of inflammation
Astrogliosis
medicine.anatomical_structure
DIFFERENTIATION
Neurology
FUNCTIONAL RECOVERY
medicine.symptom
Stem cell
Locomotion
EXPRESSION
Stromal cell
Plasticity
Inflammation
Mesenchymal Stem Cell Transplantation
03 medical and health sciences
Paracrine signalling
Rats
Nude
medicine
Regeneration
Animals
Humans
Spinal Cord Injuries
business.industry
BLOOD-BRAIN-BARRIER
Interleukin-6
Tumor Necrosis Factor-alpha
EXTRACELLULAR VESICLES
Recovery of Function
Spinal cord
medicine.disease
APOPTOTIC CASCADE
Rats
STROMAL CELLS
Neurology (clinical)
business
030217 neurology & neurosurgery
Zdroj: Journal of the Neurological Sciences, 402, 16-29. Elsevier Science
ISSN: 1878-5883
0022-510X
Popis: Spinal cord injury (SCI) is an incurable disorder with an unmet need of an effective treatment. Recently, autologous human bone marrow-derived stem cells have shown to promote functional improvement, due to their anti-inflammatory and regenerative/apocrine properties. In this study, the primary objective was to test whether a single intrathecal injection with a 100 mu L suspension of 400,000 fresh human bone marrow-derived CD34(+) and an equal number of CMOS+ stem cells (Neuro-Cells (NC)), one day after balloon-compression of the spinal cord, improves motor function and reduces secondary damage in immunodeficient rats. During the first 5 weeks after this intervention, NC significantly improved locomotor recovery and induced less injury-associated adverse events compared to vehicle-treated rats.Histological analysis showed that NC reduced astrogliosis, and apoptosis early after administration (day 4), but not at a later stage (day 56) after SCI. Proteomic studies (at day 56) pointed to the release of paracrine factors and identified proteins involved in regenerative processes. As stem cells seem to reach their effects in acute lesions by mainly suppressing (secondary) inflammation, it is thus realistic to expect a lower magnitude of their eventual beneficial effect in T-cell deficient rats, a fact reinforcing the robustness of Neuro-Cells efficacy. Taken together, this study indicates that an intrathecal instillation of Neuro-Cells holds great promise as a neuroregenerative intervention in a clinical setting with acute SCI patients.
Databáze: OpenAIRE
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