Deletion of the Transcriptional Regulator MucR in Brucella canis Affects Stress Responses and Bacterial Virulence
| Autor: | Jiali Sun, Hao Dong, Xiaowei Peng, Yufu Liu, Hui Jiang, Yu Feng, Qiaoling Li, Liangquan Zhu, Yuming Qin, Jiabo Ding |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Genetics General Veterinary Veterinary medicine Virulence Translation (biology) Brucella Biology biology.organism_classification Phenotype stress responses virulence 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Brucella canis 030220 oncology & carcinogenesis MucR SF600-1100 Transcriptional regulation RNA-Seq Gene Biogenesis |
| Zdroj: | Frontiers in Veterinary Science, Vol 8 (2021) |
| ISSN: | 2297-1769 |
| Popis: | The transcriptional regulator MucR is related to normal growth, stress responses and Brucella virulence, and affects the expression of various virulence-related genes in smooth-type Brucella strains. However, the function of MucR in the rough-type Brucella canis remains unknown. In this study, we discovered that MucR protein was involved in resistance to heat stress, iron-limitation, and various antibiotics in B. canis. In addition, the expression level of various bacterial flagellum-related genes was altered in mucR mutant strain. Deletion of this transcriptional regulator in B. canis significantly affected Brucella virulence in RAW264.7 macrophage and mice infection model. To gain insight into the genetic basis for distinctive phenotypic properties exhibited by mucR mutant strain, RNA-seq was performed and the result showed that various genes involved in translation, ribosomal structure and biogenesis, signal transduction mechanisms, energy production, and conversion were significantly differently expressed in ΔmucR strain. Overall, these studies have not only discovered the phenotype of mucR mutant strain but also preliminarily uncovered the molecular mechanism between the transcriptional regulator MucR, stress response and bacterial virulence in B. canis. |
| Databáze: | OpenAIRE |
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