Identification of the Signal Directing Tim9 and Tim10 into the Intermembrane Space of Mitochondria
| Autor: | Sabine Rospert, Thomas Ramming, Diana Stojanovski, Lena-Sophie Wenz, Dusanka Milenkovic, Agnieszka Chacinska, Judith M. Müller, Natalia Gebert, Agnes Schulze-Specking |
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| Rok vydání: | 2009 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Mitochondrial intermembrane space Recombinant Fusion Proteins Translocase of the outer membrane Molecular Sequence Data Saccharomyces cerevisiae Protein Sorting Signals Biology Mitochondrion medicine.disease_cause Mitochondrial Membrane Transport Proteins Mitochondrial membrane transport protein Leucine Mitochondrial Precursor Protein Import Complex Proteins Protein targeting medicine Amino Acid Sequence Protein Precursors Inner mitochondrial membrane Molecular Biology Membrane Proteins Articles Cell Biology Mitochondria Cell biology Protein Transport Membrane protein Mitochondrial Membranes biology.protein Intermembrane space Protein Binding |
| Zdroj: | Molecular Biology of the Cell. 20:2530-2539 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e08-11-1108 |
| Popis: | The intermembrane space of mitochondria contains the specific mitochondrial intermembrane space assembly (MIA) machinery that operates in the biogenesis pathway of precursor proteins destined to this compartment. The Mia40 component of the MIA pathway functions as a receptor and binds incoming precursors, forming an essential early intermediate in the biogenesis of intermembrane space proteins. The elements that are crucial for the association of the intermembrane space precursors with Mia40 have not been determined. In this study, we found that a region within the Tim9 and Tim10 precursors, consisting of only nine amino acid residues, functions as a signal for the engagement of substrate proteins with the Mia40 receptor. Furthermore, the signal contains sufficient information to facilitate the transfer of proteins across the outer membrane to the intermembrane space. Thus, here we have identified the mitochondrial intermembrane space sorting signal required for delivery of proteins to the mitochondrial intermembrane space. |
| Databáze: | OpenAIRE |
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