Allosteric Regulation of Histidine Kinases by Their Cognate Response Regulator Determines Cell Fate
| Autor: | Urs Jenal, Irene Wiederkehr, Tina Jaeger, Ralf Paul, Emanuele G. Biondi, Marc Folcher, Michael T. Laub, Sören Abel |
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| Rok vydání: | 2008 |
| Předmět: |
MICROBIO
Histidine Kinase Cell Allosteric regulation CELLCYCLE Biology Cell fate determination Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Allosteric Regulation Bacterial Proteins Caulobacter crescentus medicine Phosphorylation Histidine 030304 developmental biology 0303 health sciences 030306 microbiology Kinase Biochemistry Genetics and Molecular Biology(all) Histidine kinase Phosphotransferases biology.organism_classification Phosphoric Monoester Hydrolases Cell biology Crosstalk (biology) Response regulator medicine.anatomical_structure SIGNALING Signal transduction Protein Kinases 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell. 133(5) |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2008.05.018 |
| Popis: | SummaryThe two-component phosphorylation network is of critical importance for bacterial growth and physiology. Here, we address plasticity and interconnection of distinct signal transduction pathways within this network. In Caulobacter crescentus antagonistic activities of the PleC phosphatase and DivJ kinase localized at opposite cell poles control the phosphorylation state and subcellular localization of the cell fate determinator protein DivK. We show that DivK functions as an allosteric regulator that switches PleC from a phosphatase into an autokinase state and thereby mediates a cyclic di-GMP-dependent morphogenetic program. Through allosteric activation of the DivJ autokinase, DivK also stimulates its own phosphorylation and polar localization. These data suggest that DivK is the central effector of an integrated circuit that operates via spatially organized feedback loops to control asymmetry and cell fate determination in C. crescentus. Thus, single domain response regulators can facilitate crosstalk, feedback control, and long-range communication among members of the two-component network. |
| Databáze: | OpenAIRE |
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