Following the Mechanisms of Bacteriostatic versus Bactericidal Action Using Raman Spectroscopy
| Autor: | Martin Šiler, Milada Dvořáčková, Pavel Zemánek, Jan Ježek, Mojmír Šerý, Zdeněk Pilát, Ota Samek, Veronika Holá, Vladislav Krzyžánek, Silvie Bernatová, Petr Jákl, Filip Růžička |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial medicine.drug_class Antibiotics Pharmaceutical Science Microbial Sensitivity Tests 02 engineering and technology Spectrum Analysis Raman Article antibiotics Analytical Chemistry Microbiology lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry bactericidal Ciprofloxacin Staphylococcus epidermidis Drug Discovery medicine bacteriostatic Physical and Theoretical Chemistry bacteria 030304 developmental biology 0303 health sciences Bacteriostatic agent biology Clindamycin Organic Chemistry 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Anti-Bacterial Agents 3. Good health Raman spectroscopy Chemistry (miscellaneous) Molecular Medicine DNA fragmentation 0210 nano-technology Bacteria medicine.drug |
| Zdroj: | Molecules Molecules; Volume 18; Issue 11; Pages: 13188-13199 Molecules, Vol 18, Iss 11, Pp 13188-13199 (2013) |
| ISSN: | 1420-3049 |
| Popis: | Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or bacteriostatic. Bactericidal antibiotics kill the bacteria and bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/bacteriostatic data (e.g., minimum inhibitory concentrations—MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms’ “fingerprint” (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation. |
| Databáze: | OpenAIRE |
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