Fluorescent Tobacco mosaic virus-derived bio-nanoparticles for intravital two-photon imaging
| Autor: | Boudewijn van der Sanden, Annette Niehl, Sonia Boscá, Florence Appaix, Jean-François Nicoud, Frédéric Bolze, Manfred Heinlein |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Conception et application de molécules bioactives (CAMB), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Systèmes Fonctionnels, Centre National de la Recherche Scientifique (CNRS), univOAK, Archive ouverte |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Plant virus 02 engineering and technology Plant Science Biology lcsh:Plant culture Blood–brain barrier 03 medical and health sciences Two-photon excitation microscopy Tobacco mosaic virus medicine Fluorescence microscope [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BV] Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 two-photon microscopy Original Research viral nanoparticles 021001 nanoscience & nanotechnology Virology Fluorescence 030104 developmental biology medicine.anatomical_structure Biophysics intravital imaging Nanocarriers 0210 nano-technology Preclinical imaging |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2016, 6, pp.1244-1244 Frontiers in Plant Science, Vol 6 (2016) |
| ISSN: | 1664-462X |
| Popis: | Multi-photon intravital imaging has become a powerful tool to investigate the healthy and diseased brain vasculature in living animals. Although agents for multi-photon fluorescence microscopy of the microvasculature are available, issues related to stability, bioavailability, toxicity, cost or chemical adaptability remain to be solved. In particular, there is a need for highly fluorescent dyes linked to particles that do not cross the blood brain barrier (BBB) in brain diseases like tumor or stroke to estimate the functional blood supply. Plant virus particles possess a number of distinct advantages over other particles, the most important being the multi-valency of chemically addressable sites on the particle surface. This multi-valency, together with biological compatibility and inert nature, makes plant viruses ideal carriers for in vivo imaging agents. Here, we show that the well-known Tobacco mosaic virus is a suitable nanocarrier for two-photon dyes and for intravital imaging of the mouse brain vasculature. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|