| Autor: |
Christine E. Holt, Max Koppers, Roberta Cagnetta, Anaïs Bellon, Sixian Zhao, Michael S Minett, John G. Flanagan, Toshiaki Shigeoka, Lucia C. S. Wunderlich, Clemens F. Kaminski |
| Rok vydání: |
2019 |
| Předmět: |
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| Popis: |
During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors bind to distinct sets of mRNAs and RNA-binding proteins. Cue stimulation induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs in retinal axon growth cones. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by simultaneous exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and provide a general model for the rapid, localized and selective control of cue-induced translation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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