Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons

Autor: Christine E. Holt, Max Koppers, Roberta Cagnetta, Anaïs Bellon, Sixian Zhao, Michael S Minett, John G. Flanagan, Toshiaki Shigeoka, Lucia C. S. Wunderlich, Clemens F. Kaminski
Rok vydání: 2019
Předmět:
Popis: During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors bind to distinct sets of mRNAs and RNA-binding proteins. Cue stimulation induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs in retinal axon growth cones. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by simultaneous exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and provide a general model for the rapid, localized and selective control of cue-induced translation.
Databáze: OpenAIRE