Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
| Autor: | Guillermo R. Castro, German Abel Islan, Cecilia Yamil Chain, María Elena Vela, Alan Talevi, Mauricio E. Di Ianni |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Medical nanotechnology
Materials science Article Subject Nanovehicles Nanoparticle Nanotechnology Protein Corona 02 engineering and technology INGENIERÍAS Y TECNOLOGÍAS 010402 general chemistry 01 natural sciences purl.org/becyt/ford/2.10 [https] Solid lipid nanoparticle lcsh:Technology (General) General Materials Science Surface plasmon resonance Bovine serum albumin Ciencias Exactas Nanomaterials chemistry.chemical_classification Nanotecnología biology Proteins Polymer Química 021001 nanoscience & nanotechnology Nano-materiales 0104 chemical sciences Body fluids chemistry purl.org/becyt/ford/2 [https] Protein corona Drug delivery biology.protein Nanomedicine lcsh:T1-995 Nanoparticles Nanocarriers 0210 nano-technology |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP Journal of Nanomaterials, Vol 2017 (2017) |
| Popis: | The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the "protein corona" around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN. Facultad de Ciencias Exactas Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas |
| Databáze: | OpenAIRE |
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