The contribution of hormone sensitive lipase to adipose tissue lipolysis and its regulation by insulin in periparturient dairy cows

Autor: Adam L. Lock, Rahul K. Nelli, Jonas de Souza, Clarissa Strieder-Barboza, G. Andres Contreras, Jenne De Koster
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_treatment
lcsh:Medicine
Adipose tissue
Hormone-sensitive lipase
chemistry.chemical_compound
Adipocyte
Lactation
Adipocytes
Insulin
lcsh:Science
Multidisciplinary
Chemistry
Postpartum Period
FATTY LIVER
food and beverages
CONJUGATED LINOLEIC-ACID
04 agricultural and veterinary sciences
medicine.anatomical_structure
EARLY LACTATION
Lipogenesis
Female
GENE NETWORK EXPRESSION
medicine.medical_specialty
Lipolysis
TRANSITION PERIOD
Subcutaneous Fat
Article
03 medical and health sciences
Insulin resistance
LIPID-METABOLISM
Internal medicine
medicine
Animals
Veterinary Sciences
SUBCLINICAL KETOSIS
ADIPOCYTE SIZE
lcsh:R
0402 animal and dairy science
Biology and Life Sciences
BODY CONDITION SCORE
Sterol Esterase
medicine.disease
040201 dairy & animal science
030104 developmental biology
Endocrinology
HOLSTEIN COWS
Cattle
lcsh:Q
Zdroj: SCIENTIFIC REPORTS
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
ISSN: 2045-2322
Popis: Hormone sensitive lipase (HSL) activation is part of the metabolic adaptations to the negative energy balance common to the mammalian periparturient period. This study determined HSL contribution to adipose tissue (AT) lipolysis and how insulin regulates its activity in periparturient dairy cows. Subcutaneous AT (SCAT) samples were collected at 11 d prepartum (dry) and 11 (fresh) and 24 d (lactation) postpartum. Basal and stimulated lipolysis (ISO) responses were determined using explant cultures. HSL contribution to lipolysis was assessed using an HSL inhibitor (CAY). Basal lipolysis was higher in SCAT at dry compared with fresh. CAY inhibited basal lipolysis negligibly at dry, but at fresh and lactation it reduced basal lipolysis by 36.1 ± 4.51% and 43.1 ± 4.83%, respectively. Insulin inhibited lipolysis more pronouncedly in dry compared to fresh. Results demonstrate that HSL contribution to basal lipolysis is negligible prepartum. However, HSL is a major driver of SCAT lipolytic responses postpartum. Lower basal lipolysis postpartum suggests that reduced lipogenesis is an important contributor to fatty acid release from SCAT. Loss of adipocyte sensitivity to the antilipolytic action of insulin develops in the early lactation period and supports a state of insulin resistance in AT of cows during the first month postpartum.
Databáze: OpenAIRE
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