Salting-out assisted liquid-liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples
| Autor: | Moi Me Chong, Ker Yin Chew, Mazidatulakmam Miskam, Nur Hidayah Sazali, Anas Alshishani, Bahruddin Saad |
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| Rok vydání: | 2019 |
| Předmět: |
Vitamin
vitamin d3 Multidisciplinary Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Extraction (chemistry) milk samples 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound Liquid–liquid extraction Salting out lcsh:Q hplc-dad lcsh:Science Hplc dad Research Article salting-out liquid–liquid extraction |
| Zdroj: | Royal Society Open Science, Vol 6, Iss 8 (2019) Royal Society Open Science |
| ISSN: | 2054-5703 |
| Popis: | In this study, salting-out assisted liquid–liquid extraction (SALLE) as a simple and efficient extraction technique followed by high-performance liquid chromatography (HPLC) was employed for the determination of vitamin D3 in milk samples. The sample treatment is based on the use of water-miscible acetonitrile as the extractant and acetonitrile phase separation under high-salt conditions. Under the optimum conditions, acetonitrile and ammonium sulfate were used as the extraction solvent and salting-out agent, respectively. The vitamin D3 extract was separated using Hypersil ODS (250x i.d 4.6 mm, 5 µm) HPLC column that was coupled with diode array detector. Vitamin D2 was used as internal standard (IS) to offset any variations in chromatographic conditions. The vitamin D3 and the IS were eluted in 18 min. Good linearity ( r 2 > 0.99) was obtained within the range of 25–600 ng g −1 with the limit of detection of 15 ng g −1 and limit of quantification of 25 ng g −1 . The validated method was applied for the determination of vitamin D3 in milk samples. The recoveries for spiked samples were from 94.4 to 113.5%. |
| Databáze: | OpenAIRE |
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