Adsorption of mesenchymal stem cells and cortical neural stem cells on carbon nanotube/polycarbonate urethane
| Autor: | Jong Youl Kim, Thomas J. Webster, Jong Eun Lee, Yoonmi Nho, Dongwoo Khang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Materials science
Cell Survival Scanning electron microscope Neurogenesis Immunocytochemistry Biomedical Engineering Medicine (miscellaneous) Bioengineering Nanotechnology Carbon nanotube Development Urethane law.invention Mice law Neurosphere Cell Adhesion Extracellular Animals Humans General Materials Science Cells Cultured Neurons Polycarboxylate Cement Nanotubes Carbon Mesenchymal stem cell Mesenchymal Stem Cells Neural stem cell Biophysics Adsorption Stem cell |
| Zdroj: | Nanomedicine. 5:409-417 |
| ISSN: | 1748-6963 1743-5889 |
| Popis: | Background & aim: Carbon nanotubes (CNTs) are a promising material for implantation due to the fact that CNTs are conductive and have nanostructured dimensions that mimic the 3D structure of proteins found in extracellular matrices. We have investigated whether the CNTs interact with various types of stem cells and either selectively enhance survival of stem cells or not. Materials & methods: CNTs used in the experiments are aligned with polycarbonate urethane. Experiments were carried out using mesenchymal stem cells (MSCs) and cortical derived neurospheres. Immunocytochemistry and scanning electron microscopic analysis were performed for determining the favorable surface material (either CNT or polycarbonate urethane array) for cell survival. Results: It was demonstrated that the MSCs and the neurosphere of cortex-derived neural stem cells (NSCs) grew on the CNT array and both MSCs and NSCs interacted with the aligned CNTs. The results suggest that CNTs assist in the proliferation of MSCs and aid differentiation of cortex-derived NSCs. Conclusion: CNTs may be a novel biocompatible nanophase material with the potential for aiding neuron differentiation. |
| Databáze: | OpenAIRE |
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