Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types
| Autor: | Manfred Stolte, Sabine Krüger-Krasagakes, Anette Schmitt-Gräff, Harald Stein, Ernst Otto Riecken, Ian F. C. McKenzie, Cees P. H. J. Verstijnen, Enno Klussmann, Monika Blank, Christoph Hanski, Gudrun Bornhoeft, Pei-Xiang Xing |
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| Rok vydání: | 1994 |
| Předmět: |
Adenoma
Cancer Research Pathology medicine.medical_specialty Molecular Sequence Data Gene Expression Mucin 2 Biology Polymerase Chain Reaction digestive system Epitope Diagnosis Differential Immunoenzyme Techniques Adenomatous Polyps Gene expression Adenoma Villous Biomarkers Tumor medicine Carcinoma Humans RNA Messenger DNA Primers Mucin-2 Base Sequence Mucin Mucins respiratory system medicine.disease Adenocarcinoma Mucinous digestive system diseases Neoplasm Proteins Oncology Immunohistochemistry Adenocarcinoma Colorectal Neoplasms Precancerous Conditions |
| Zdroj: | International Journal of Cancer. 59:301-306 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910590302 |
| Popis: | The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non-mucinous type. The latter were subdivided into carcinomas originating from the adenoma-carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti-MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21% of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21% and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non-malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over-expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non-mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2. |
| Databáze: | OpenAIRE |
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