Conformational transition in SPR experiments: impact of spacer length, immobilization mode and aptamer density on signal sign and amplitude

Autor:	Marina Pons, Marine Perenon, Hugues Bonnet, Emilie Gillon, Celio Vallée, Liliane Coche-Guérente, Eric Defrancq, Nicolas Spinelli, Angeline Van der Heyden, Jérôme Dejeu
Rok vydání:	2022
Předmět:	Oligonucleotides Electrochemistry Environmental Chemistry Calorimetry Surface Plasmon Resonance Ligands Biochemistry Spectroscopy Analytical Chemistry
Zdroj:	The Analyst. 147:4197-4205
ISSN:	1364-5528 0003-2654
DOI:	10.1039/d2an00824f
Popis:	Surface plasmon resonance (SPR) is an optical, real-time and label-free technique which represents a standard to study biomolecular interactions. While SPR signals are usually positive upon recognition, a few cases of negative signals have been reported because of significant conformational transition of the receptor upon the recognition of the target. In this study, we reported on the observation of negative or null SPR signals for an aptamer recognition with its low molecular weight target. The introduction of a spacer group for the aptamer immobilization led to a null SPR signal despite the device sensitivity and effective target recognition (a
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a240738ad7ada4e96fe12d433cad754 https://doi.org/10.1039/d2an00824f Zobrazit plný text záznamu