Adult neurogenesis and specific replacement of interneuron subtypes in the mouse main olfactory bulb

Autor: Nathaniel N. Urban, Joshua A. Bagley, Greg LaRocca, Daniel A. Jimenez
Rok vydání: 2007
Předmět:
Calbindins
Tyrosine 3-Monooxygenase
Interneuron
Rostral migratory stream
Subventricular zone
Nerve Tissue Proteins
S100 Calcium Binding Protein beta Subunit
Biology
lcsh:RC321-571
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
S100 Calcium Binding Protein G
0302 clinical medicine
Neuroblast
Cell Movement
Interneurons
medicine
Animals
Nerve Growth Factors
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Cellular Senescence
gamma-Aminobutyric Acid
030304 developmental biology
0303 health sciences
General Neuroscience
Calcium-Binding Proteins
S100 Proteins
lcsh:QP351-495
Neurogenesis
Nuclear Proteins
Granule cell
Olfactory Bulb
Olfactory bulb
DNA-Binding Proteins
Mice
Inbred C57BL
Adult Stem Cells
lcsh:Neurophysiology and neuropsychology
medicine.anatomical_structure
Bromodeoxyuridine
Microscopy
Fluorescence
nervous system
Calbindin 2
Calreticulin
Neuroscience
Biomarkers
030217 neurology & neurosurgery
Research Article
Adult stem cell
Zdroj: BMC Neuroscience, Vol 8, Iss 1, p 92 (2007)
BMC Neuroscience
ISSN: 1471-2202
Popis: Background New neurons are generated in the adult brain from stem cells found in the subventricular zone (SVZ). These cells proliferate in the SVZ, generating neuroblasts which then migrate to the main olfactory bulb (MOB), ending their migration in the glomerular layer (GLL) and the granule cell layer (GCL) of the MOB. Neuronal populations in these layers undergo turnover throughout life, but whether all neuronal subtypes found in these areas are replaced and when neurons begin to express subtype-specific markers is not known. Results Here we use BrdU injections and immunohistochemistry against (calretinin, calbindin, N-copein, tyrosine hydroxylase and GABA) and show that adult-generated neurons express markers of all major subtypes of neurons in the GLL and GCL. Moreover, the fractions of new neurons that express subtype-specific markers at 40 and 75 days post BrdU injection are very similar to the fractions of all neurons expressing these markers. We also show that many neurons in the glomerular layer do not express NeuN, but are readily and specifically labeled by the fluorescent nissl stain Neurotrace. Conclusion The expression of neuronal subtype-specific markers by new neurons in the GLL and GCL changes rapidly during the period from 14–40 days after BrdU injection before reaching adult levels. This period may represent a critical window for cell fate specification similar to that observed for neuronal survival.
Databáze: OpenAIRE
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