Metabolic changes associated with selenium deficiency in mice
| Autor: | Michelle L. Villemaire, Linda E. Sandercock, Beata Mickiewicz, Hans J. Vogel, Frank R. Jirik |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Metabolite chemistry.chemical_element Inflammation Biology General Biochemistry Genetics and Molecular Biology Antioxidants Gas Chromatography-Mass Spectrometry Biomaterials Biological pathway chemistry.chemical_compound Mice Selenium Metabolomics Biosynthesis Selenium deficiency Internal medicine medicine Animals Amino Acids Pyruvates Selenoproteins Nuclear Magnetic Resonance Biomolecular Metals and Alloys Metabolism medicine.disease Mice Inbred C57BL Endocrinology chemistry medicine.symptom General Agricultural and Biological Sciences Oxidation-Reduction |
| Zdroj: | Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 27(6) |
| ISSN: | 1572-8773 |
| Popis: | Selenium (Se), which is a central component for the biosynthesis and functionality of selenoproteins, plays an important role in the anti-oxidative response, reproduction, thyroid hormone metabolism and the protection from infection and inflammation. However, dietary Se effects have not well been established to date and the available studies often present contradictory results. To obtain a better understanding of Se intake and its influence on the metabolism of living systems, we have utilized a metabolomics approach to gain insight into the specific metabolic alterations caused by Se deficiency in mice. Serum samples were collected from two groups of C57BL/6 mice: an experimental group which was fed a Se-deficient diet and controls consuming normal chow. The samples were analyzed by (1)H nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. The resulting metabolite data were examined separately for both analytical methods and in a combined manner. By applying multivariate statistical analysis we were able to distinguish the two groups and detect a metabolite pattern associated with Se deficiency. We found that the concentrations of 15 metabolites significantly changed in serum samples collected from Se-deficient mice when compared to the controls. Many of the perturbed biological pathways pointed towards compensatory mechanisms during Se deficiency and were associated with amino acid metabolism. Our findings show that a metabolomics approach may be applied to identify the metabolic impact of Se and reveal the most impaired biological pathways as well as induced regulatory mechanisms during Se deficiency. |
| Databáze: | OpenAIRE |
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