Firefly Luciferase Generates Two Low-Molecular-Weight Light-Emitting Species
| Autor: | Frédéric Brau, Jean-Claude Bernengo, Kyung-Lyum Min, Jean-Paul Steghens |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Binding Sites Size-exclusion chromatography Biophysics Analytical chemistry Cell Biology Firefly Luciferin Hydrogen-Ion Concentration Biochemistry Coleoptera Molecular Weight Wavelength Adenosine Triphosphate Deoxyadenine Nucleotides chemistry Chromatography detector Luminescent Measurements Animals Bioluminescence Luciferase Nucleotide Luciferases Luminescence Molecular Biology Common emitter |
| Zdroj: | Biochemical and Biophysical Research Communications. 270:247-253 |
| ISSN: | 0006-291X |
| Popis: | A bioluminescent D-luciferin-luciferase mixture is separated by gel filtration during the time course of the reaction. A simultaneous analysis with an UV-visible diode array detector and an on-line luminometer gives nonsuperimposable chromatograms. Luminescence recordings display three peaks, one associated with the enzyme (light-emitting species 1: LES(1)), and two other species free from the luciferase: LES(2), with a luciferyl-adenylate-like spectrum and LES(3). Production of these two species is nucleotide (ATP or 2'-dATP)- and pH-dependent. The chromatographic data presented here could lead to reconsideration of the generally assumed emission mechanism, which involves one emitter only. It could also suggest that each free emitting species is related to a colour of emission corresponding to the two defined wavelengths previously described ( approximately 575 and approximately 620 nm). |
| Databáze: | OpenAIRE |
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