Detection of two hypervariable (ATTTT)n loci in the human genome
| Autor: | Christine Luckenbach, C. Dürr, Horst Ritter, Anke Hinney |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Molecular Sequence Data Clinical Biochemistry Locus (genetics) Biology Biochemistry Analytical Chemistry law.invention symbols.namesake law Humans Child Allele frequency Polymerase chain reaction Repetitive Sequences Nucleic Acid Southern blot Genetics Base Sequence Genome Human Genetic Variation DNA Fingerprinting Pedigree Hypervariable region Mendelian inheritance symbols Female Human genome Oligonucleotide Probes Oligomer restriction |
| Zdroj: | Electrophoresis. 16:719-721 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | Object of this investigation was the isolation of a single-locus probe from a multi-locus fingerprint. Individual specific multi-locus fingerprints in man were generated by using the oligonucleotide probe (ATTTT)5. An isolated (ATTTT)5-positive DNA fragment was analyzed using polymerase chain reaction (PCR) cycle-sequencing and nonradioactive direct-blotting electrophoresis. A digoxigenated oligonucleotide synthesized according to this sequence was used as a single-locus probe. Two hypervariable loci were detected on Southern blots. Formal genetic investigations for the two loci were performed in order to estimate the allele frequencies. Locus 1 shows an individual-specific banding pattern with an autosomal-codominant inheritance and can be used for forensic investigations. Locus 2 also represents a polymorphic pattern, but the inheritance is not according to the Mendelian rules. Probably we have detected a highly mutagenic locus in the human genome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text