Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.)
| Autor: | Lara Padovan, Ana Maria Benko-Iseppon, Sergio Crovella, Ludovica Segat, Luis Carlos Belarmino, Maria Clara Pestana-Calsa, Akio Kido Ederson, Tercilio Calsa, Alessandro Tossi, Lucas André Cavalcanti Brandão, Valesca Pandolfi, Rafael Lima Guimarães |
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| Přispěvatelé: | Padovan, Lara, Segat, Ludovica, Tossi, Alessandro, T., Calsa, A. K., Ederson, L., Brandao, R. L., Guimarãe, V., Pandolfi, M. C., Pestana Calsa, L. C., Belarmino, A. M., Benko Iseppon, Crovella, Sergio |
| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular antimicrobial peptide plant defensin expression Protein Conformation Plant defensin Antimicrobial peptides Comovirus Molecular Sequence Data Biochemistry Microbiology Vigna Defensins Structural Biology Sequence Analysis Protein Botany Amino Acid Sequence Phaseoleae Defensin Gene Plant Proteins integumentary system biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction fungi Cowpea mosaic virus food and beverages Fabaceae General Medicine respiratory system bacterial infections and mycoses biology.organism_classification RNA splicing Sequence Alignment |
| Zdroj: | Protein and peptide letters. 17(3) |
| ISSN: | 1875-5305 |
| Popis: | Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna un-guiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested. |
| Databáze: | OpenAIRE |
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