Studies on Differentiation without Cleavage in Chaetopterus: Effects of Inhibition of DNA Synthesis with Aphidicolin
| Autor: | B. De Petrocellis, J. Bracket, Henri Alexandre |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
DNA Replication
Aphidicolin Cancer Research Time Factors animal structures DNA polymerase DNA -- biosynthesis Chaetopterus chemistry.chemical_compound Larva -- drug effects Animals Molecular Biology Diterpenes -- pharmacology DNA synthesis biology Pseudocleavage DNA Replication -- drug effects DNA replication food and beverages Cell Differentiation Polychaeta DNA Cell Biology Sciences bio-médicales et agricoles biology.organism_classification Molecular biology Nuclear DNA Cell Differentiation -- drug effects chemistry Larva embryonic structures biology.protein Polychaeta -- metabolism -- physiology Diterpenes Developmental Biology |
| Zdroj: | Differentiation, 19 (1 |
| ISSN: | 0301-4681 |
| DOI: | 10.1111/j.1432-0436.1981.tb01126.x |
| Popis: | The effects of aphidicolin - a powerful inhibitor of DNA polymerase alpha and of DNA replication - on normal development and on differentiation without cleavage of Chaetopterus eggs have been studied with cytological, cytochemical, and biochemical methods. The experiments show that the initial period of pseudocleavage can take place in the absence of nuclear DNA synthesis, but further development (segregation, hatching, ciliation) requires DNA synthesis. However ciliated unicellular larvae can be obtained under conditions where the DNA content of the embryos in only 40% of the controls. In fertilized eggs, aphidicolin immediately stops cleavage. The significance of these results is discussed. Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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