Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations
| Autor: | Nan Peng, Yingjun Li, Qunxin She, Tao Liu, Saifu Pan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Application Archaeal Proteins Biology Genome engineering 03 medical and health sciences chemistry.chemical_compound Genetic Bacterial Proteins RNA interference CRISPR Gene silencing Clustered Regularly Interspaced Short Palindromic Repeats Gene Silencing CRISPR-Cas Gene Manipulations System Genetics Gene Editing Introduction Bacteria Base Sequence RNA General Medicine Endonucleases Archaea 030104 developmental biology chemistry Nucleic acid CRISPR-Cas Systems DNA RNA Guide Kinetoplastida |
| Zdroj: | Current Issues in Molecular Biology Volume 19 Issue 1 Pages 1-14 |
| ISSN: | 1467-3045 |
| Popis: | Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing. |
| Databáze: | OpenAIRE |
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