Antibacterial Activity of Long-Chain Polyunsaturated Fatty Acids against Propionibacterium acnes and Staphylococcus aureus
| Autor: | Keelan C. Lawlor, Andrew P. Desbois |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
benzoyl peroxide
acne vulgaris C20:5n-3 combination therapy docosahexaenoic acid (DHA) natural products omega-3 skin infection synergy Staphylococcus aureus Pharmaceutical Science Microbial Sensitivity Tests medicine.disease_cause Article Microbiology Propionibacterium acnes Hydroxyeicosatetraenoic Acids Drug Discovery medicine lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) Acne Skin chemistry.chemical_classification biology Neomycin medicine.disease biology.organism_classification Antimicrobial Eicosapentaenoic acid Anti-Bacterial Agents Eicosapentaenoic Acid lcsh:Biology (General) chemistry Fatty Acids Unsaturated Antibacterial activity Polyunsaturated fatty acid medicine.drug |
| Zdroj: | Marine Drugs, Vol 11, Iss 11, Pp 4544-4557 (2013) Marine Drugs Marine Drugs; Volume 11; Issue 11; Pages: 4544-4557 |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md11114544 |
| Popis: | New compounds are needed to treat acne and superficial infections caused by Propionibacterium acnes and Staphylococcus aureus due to the reduced effectiveness of agents used at present. Long-chain polyunsaturated fatty acids (LC-PUFAs) are attracting attention as potential new topical treatments for Gram-positive infections due to their antimicrobial potency and anti-inflammatory properties. This present study aimed to investigate the antimicrobial effects of six LC-PUFAs against P. acnes and S. aureus to evaluate their potential to treat infections caused by these pathogens. Minimum inhibitory concentrations were determined against P. acnes and S. aureus, and the LC-PUFAs were found to inhibit bacterial growth at 32–1024 mg/L. Generally, P. acnes was more susceptible to the growth inhibitory actions of LC-PUFAs, but these compounds were bactericidal only for S. aureus. This is the first report of antibacterial activity attributed to 15-hydroxyeicosapentaenoic acid (15-OHEPA) and 15-hydroxyeicosatrienoic acid (HETrE), while the anti-P. acnes effects of the six LC-PUFAs used herein are novel observations. During exposure to the LC-PUFAs, S. aureus cells were killed within 15–30 min. Checkerboard assays demonstrated that the LC-PUFAs did not antagonise the antimicrobial potency of clinical agents used presently against P. acnes and S. aureus. However, importantly, synergistic interactions against S. aureus were detected for combinations of benzoyl peroxide with 15-OHEPA, dihomo-γ-linolenic acid (DGLA) and HETrE; and neomycin with 15-OHEPA, DGLA, eicosapentaenoic acid, γ-linolenic acid and HETrE. In conclusion, LC-PUFAs warrant further evaluation as possible new agents to treat skin infections caused by P. acnes and S. aureus, especially in synergistic combinations with antimicrobial agents already used clinically. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|