| Autor: |
Zhang, Blake, Moran, Anne, Bailey, Charles, Rasko, John, Holst, Jeff, Wang, Qian |
| Rok vydání: |
2019 |
| DOI: |
10.6084/m9.figshare.9108863 |
| Popis: |
Figure S1. A, LAT1 expression after EGF treatment in PC-3 cells. GAPDH was used as loading control. B, leucine uptake in PC-3 cells after JPH203 treatment in the absence or presence of EGF for 30 min. Two tailed Student’s t-test was performed. Data are the mean ± SEM, n = 4. C, GAPDH is examined in cell surface fraction in LNCaP cells. Cell lysates of LNCaP cells are used as positive control. D and E, Ubiquitin and LAT3 were examined after immunoprecipitation with anti-LAT3 in the presence or absence of EGF in LNCaP (D) and PC-3 (E) cells. Ratio of IB: ubiquitin expression level is shown relative to Input: LAT3. F, LAT3 expression levels were examined in the absence or presence of MG132 in EGF treated LNCaP or PC-3 cells. GAPDH was used as loading control. Protein expression levels were normalised to loading control. (PDF 1131 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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