A network of local and redundant gene regulation governs Arabidopsis seed maturation
| Autor: | Jérôme Giraudat, Alexandra To, Martine Devic, Jocelyne Guilleminot, François Parcy, Christiane Valon, Gil Savino |
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| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Universitat Politècnica de València (UPV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
anthocyanin biosynthesis Arabidopsis thaliana Mutant Arabidopsis plant Plant Science phytohormone medicine.disease_cause 01 natural sciences MESH: CCAAT-Enhancer-Binding Proteins chemistry.chemical_compound Gene Expression Regulation Plant MESH: Arabidopsis Abscisic acid Research Articles In Situ Hybridization transgenic plant Genetics Regulation of gene expression 0303 health sciences Mutation biology MESH: Transcription Factors abi3 Plants Genetically Modified storage proteins Seeds Fus3 fus3 Cotyledon MESH: Cotyledon MESH: Mutation Recombinant Fusion Proteins pigments MESH: Arabidopsis Proteins dessication tolerance [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences MESH: In Situ Hybridization medicine MESH: Recombinant Fusion Proteins [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology mutant MESH: Gene Expression Regulation Plant Gene 030304 developmental biology abscisic acid sensitivity Arabidopsis Proteins maturation chlorophyll degradation Cell Biology biology.organism_classification chemistry MESH: Plants Genetically Modified MESH: Seeds lec1 lec2 CCAAT-Enhancer-Binding Proteins gene expression gene regulation seed Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Cell Plant Cell, American Society of Plant Biologists, 2006, 18, pp.1642-1651. ⟨10.1105/tpc.105.039925⟩ The Plant cell The Plant cell, 2006, 18, pp.1642-1651. ⟨10.1105/tpc.105.039925⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2006, 18, pp.1642-1651. ⟨10.1105/tpc.105.039925⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2006, 18 (7), pp.1642-51. ⟨10.1105/tpc.105.039925⟩ |
| ISSN: | 1040-4651 1532-298X |
| Popis: | In Arabidopsis thaliana, four major regulators (ABSCISIC ACID INSENSITIVE3 [ABI3], FUSCA3 [FUS3], LEAFY COTYLEDON1 [LEC1], and LEC2) control most aspects of seed maturation, such as accumulation of storage compounds, cotyledon identity, acquisition of desiccation tolerance, and dormancy. The molecular basis for complex genetic interactions among these regulators is poorly understood. By analyzing ABI3 and FUS3 expression in various single, double, and triple maturation mutants, we have identified multiple regulatory links among all four genes. We found that one of the major roles of LEC2 was to upregulate FUS3 and ABI3. The lec2 mutation is responsible for a dramatic decrease in ABI3 and FUS3 expression, and most lec2 phenotypes can be rescued by ABI3 or FUS3 constitutive expression. In addition, ABI3 and FUS3 positively regulate themselves and each other, thereby forming feedback loops essential for their sustained and uniform expression in the embryo. Finally, LEC1 also positively regulates ABI3 and FUS3 in the cotyledons. Most of the genetic controls discovered were found to be local and redundant, explaining why they had previously been overlooked. This works establishes a genetic framework for seed maturation, organizing the key regulators of this process into a hierarchical network. In addition, it offers a molecular explanation for the puzzling variable features of lec2 mutant embryos. |
| Databáze: | OpenAIRE |
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