A new objective method for scoring human sperm hyperactivation based on head axis angle deviation
| Autor: | Amnon Makler, Franco Dondero, Michele Delfino, Tiziana Rossi, Fernando Mazzilli |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | International Journal of Andrology. 24:189-196 |
| ISSN: | 1365-2605 0105-6263 |
| DOI: | 10.1046/j.1365-2605.2001.00271.x |
| Popis: | The aims of the present study were, first to develop a new method for evaluating sperm hyperactivation (HA) based on sperm head axis angle deviation, and second to apply this method in scoring selected sperm populations during capacitation. This was made possible by improving our original superimposed image analysis system (SIAS). The new option on the system enables us to determine the real angular deviation of the entire head in successive superimposed frames. The entire procedure for each spermatozoon requires 10-20 sec, according to the frame/rate utilized. A clear cut-off between the values of hyperactivated, transitional and non-hyperactivated spermatozoa was found at 11 and 21 frames/sec. However, at 6 frames/sec, a partial overlap between the three classes was found. We defined sperm activity as follows. At 11 frames/sec, hyperactivated: angle sumor = 405 degrees; transitional: 200 degreesor = angle sum405 degrees. At 21 frames/sec, hyperactivated: angle sumor = 760 degrees; transitional: 350 degreesor = angle sum760 degrees. The results, in agreement with previous research, show the peaks of activity at 3 h, dropping to a minimum at 6 h. This phenomenon was significantly more evident in fertile compared with subfertile semen samples. |
| Databáze: | OpenAIRE |
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