Mesoporous silica nanoparticles trigger mitophagy in endothelial cells and perturb neuronal network activity in a size- and time-dependent manner
| Autor: | Maria Tringali, Francesca Gullo, Stefania Minniti, Ennio Tasciotti, Francesca Re, Andrea Becchetti, Antonina Orlando, Emanuela Cazzaniga, Francesca Taraballi |
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| Přispěvatelé: | Orlando, A, Cazzaniga, E, Tringali, M, Gullo, F, Becchetti, A, Minniti, S, Taraballi, F, Tasciotti, E, Re, F |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mesoporous silica nanoparticle Cell Pharmaceutical Science 02 engineering and technology chemistry.chemical_compound Drug Delivery Systems Endothelial cell International Journal of Nanomedicine Mitophagy Drug Discovery Original Research Neurons MEA Brain General Medicine Silicon Dioxide 021001 nanoscience & nanotechnology Enzymes medicine.anatomical_structure Drug delivery Toxicity Electrophoresis Polyacrylamide Gel 0210 nano-technology Materials science Cell Survival Biophysics Nanotechnology Bioengineering Nitric Oxide Cell Line Nitric oxide Biomaterials 03 medical and health sciences Autophagy Human Umbilical Vein Endothelial Cells medicine Humans Nanotoxicity Viability assay mesoporous silica nanoparticles Dose-Response Relationship Drug Cell Membrane Organic Chemistry Endothelial Cells Neuron Biomaterial In vitro 030104 developmental biology chemistry Biophysic Nanotoxicology Nanoparticles |
| Zdroj: | International Journal of Nanomedicine |
| Popis: | Antonina Orlando,1 Emanuela Cazzaniga,1 Maria Tringali,2 Francesca Gullo,3 Andrea Becchetti,3 Stefania Minniti,1 Francesca Taraballi,4,5 Ennio Tasciotti,4,5 Francesca Re1 1Nanomedicine Center, School of Medicine and Surgery, University of Milano-Bicocca, Monza, 2Department of Environmental Sciences, 3Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy; 4Center for Biomimetic Medicine, Houston Methodist Research Institute (HMRI), 5Department of Orthopedics and Sports Medicine, Houston Methodist Hospital, Houston, TX, USA Purpose: Mesoporous silica nanoparticles (MSNPs) are excellent candidates for biomedical applications and drug delivery to different human body areas, the brain included. Although toxicity at cellular level has been investigated, we are still far from using MSNPs in the clinic, because the mechanisms involved in the cellular responses activated by MSNPs have not yet been elucidated.Materials and methods: This study used an in vitro multiparametric approach to clarify relationships among size, dose, and time of exposure of MSNPs (0.05–1 mg/mL dose range), and cellular responses by analyzing the morphology, viability, and functionality of human vascular endothelial cells and neurons.Results: The results showed that 24 hours of exposure of endothelial cells to 250 nm MSNPs exerted higher toxicity in terms of mitochondrial activity and membrane integrity than 30 nm MSN at the same dose. This was due to induced cell autophagy (in particular mitophagy), probably consequent to MSNP cellular uptake (>20%). Interestingly, after 24 hours of treatment with 30 nm MSNPs, very low MSNP uptake ( |
| Databáze: | OpenAIRE |
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